个人简介

殷雷，教授，博士生导师，重庆医科大学附属儿童医院国家临床医学研究中心，国家高层次青年人才，重庆高层次引进人才。主要从事抗原识别及基因编辑医药挖掘，发表学术论文近50篇。以第一作者/通讯作者在《Immunity》、《Nature communications》（2篇）、《Genome Biology》、《PNAS》（2篇）、《Science Advances》、《EClinicalMedicine》、《Molecular Therapy》、《Plos Biology》（2篇）等专业TOP期刊上发表研究成果30余篇，3篇配发特邀评论重点介绍，一篇入选杂志封面并入选ESI高被引。主持国家重点重大课题和国家自然科学基金多项，获省部级科技进步奖一等奖，担任中国生物物理学会人工智能生物学分会委员，武汉生物物理学会理事长，国家重点研发计划二审专家等。近5年来，围绕抗原和TCR/BCR的筛选鉴定、单细胞TCR/BCR和空间TCR/BCR大数据研究、分子互作和结构分析、低脱靶多基因编辑和相关医药挖掘等方面建立了新的技术平台体系，开发出多个自主产权的抗原识别增强新方法、低脱靶Cas12a基因编辑酶和小分子候选药物,授权专利5项，转化一项。组建抗原识别数字平台和基因编辑平台，每年招收硕博士2-4人和医院固定研究人员1-2人。

教育背景及工作简历

1998-2002武汉大学生命科学学院基地班专业学士

2002-2007中国科学院生物物理研究所生物大分子国家重点实验室硕士/博士

2008-2013美国霍华德休斯医学研究所（HHMI） 研究助理

导师：John Kappler院士Philippa Marrack院士（英国皇家学会会员）

2014-2024武汉大学生命科学学院病毒学国家重点实验室教授博导

2025-至今重庆医科大学附属儿童医院 国家临床医学研究中心 教授 博导

荣誉与获奖

国家海外高层次青年人才,广东省科技进步奖一等奖,重庆市高层次人才

国内外学术团体任职

武汉生物物理学会理事长

中国生物物理学会人工智能生物学分会委员

基金项目

1.新型病原体模式识别受体、信号分子及调控分子的发现、作用机制与结构研究。国家重大科学研究计划（973）课题，课题负责人，结题

2.T细胞受体识别肿瘤多肽疫苗的结构和功能研究。国家自然科学基金，负责人，结题

3.高效多肽疫苗激活T细胞抗肿瘤的复合物结构研究及分子机制。国家自然科学基金，负责人，结题

4.银屑病自身免疫原的T细胞受体识别。国家自然科学基金，负责人，结题

5.Cas12aPAM识别严格性和脱靶的结构功能关系研究。国家自然科学基金，负责人，在研

6.宿主抗结核免疫相关关键生物大分子的调控机制。国家重点研发计划，骨干,在研

7.小型高保真Cas12a的结构基础和改造设计。国家自然科学基金,负责人，在研

代表性论文(*通讯作者，#共同第一作者)

1.Chen P, Wu Y, Wang H, Liu H, Zhou J, Lei J, Sun Z, Yin L*. Highly parallel profiling of the activities and specificities of Cas12a variants in human cells. Nat Commun. 2025 Mar 28;16(1):3022

2.Chen Q, Pang M, Chen P, Zhou Z, Lei J, He B, Sun Z, Paek C, Jing B, Wu Y, Liu S, Chen Y, Yin L*. High-fidelity CRISPR/Cas12a dual-crRNA screening reveals novel synergistic interactions in hepatocellular carcinoma. Clin Transl Med. 2024 Jul;14(7):e1758

3.Wang H, Zhou J, Lei J, Mo G, Wu Y, Liu H, Pang Z, Du M, Zhou Z, Paek C, Sun Z, Chen Y, Wang Y*, Chen P*, Yin L*. Engineering of a compact, high-fidelity EbCas12a variant that can be packaged with its crRNA into an all-in-one AAV vector delivery system. PLoS Biol. 2024 May 30;22(5):e3002619.

4.Fang XX, Wei P, Zhao K, Sheng ZC, Song BL*, Yin L*, Luo J*. Fatty acid-binding proteins 3, 7, and 8 bind cholesterol and facilitate its egress from lysosomes. J Cell Biol. 2024 Apr 1;223(4):e202211062.

5.Chen P, Zhou J, Liu H, Zhou E, He B, Wu Y, Wang H, Sun Z, Paek C, Lei J, Chen Y*, Zhang X*, Yin L*. Engineering of Cas12a nuclease variants with enhanced genome-editing specificity. PLoS Biol. 2024 Mar 14;22(3):e3002514.

6.Wang Y, Sun Z, Ping J, Tang J, He B, Chang T, Zhou Q, Yuan S, Tang Z, Li X, Lu Y, He R, He X, Liu Z*, Yin L*, Wu N*. Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function. Nat Commun. 2023 Nov 4;14(1):7075.

7.Zhao W, Lei J, Ke S, Chen Y, Xiao J, Tang Z, Wang L, Ren Y, Alnaggar M, Qiu H, Shi W, Yin L*, Chen Y*. Fecal microbiota transplantation plus tislelizumab and fruquintinib in refractory microsatellite stable metastatic colorectal cancer: an open-label, single-arm, phase II trial (RENMIN-215). EClinicalMedicine. 2023 Nov 14;66:102315.

8.Zhou J, Chen P, Wang H, Liu H, Li Y, Zhang Y, Wu Y, Paek C, Sun Z, Lei J, Yin L*. Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition. Molecular Therapy. 2022，30（1）

9.Wei P, Jordan K, Buhrman J, Lei J, Deng H, Marrack P, Dai S, Kappler JW*, Slansky J*, Yin L*. Structures suggest an approach for converting weak self-peptide tumor antigens into superagonists for CD8 T cell in cancer. PNAS. 2021 Jun 8;118(23):e2100588118.

10.Bai P, Zhou Q, Wei P, Bai H, Chan SK, Kappler JW, Marrack P, Yin L*. Rational discovery of a cancer neoepitope harboring the KRAS G12D driver mutation. Sci China Life Sci. 2021 Dec;64(12):2144-2152

11.Qu Z, Zhou J, Zhou Y, Xie Y, Jiang Y, Wu J, Luo Z, Liu G, Yin L*, Zhang XL*. Mycobacterial EST12 activates a RACK1-NLRP3-gasdermin D pyroptosis immune pathway. Science Advances. 2020 Oct 23;6(43):eaba4733.

12.Chen P, Zhou J, Wan Y, Liu H, Li Y, Liu Z, Wang H, Lei J, Zhao K, Zhang Y, Wang Y, Zhang X, Yin L*. A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing. Genome Biology. 2020 Mar 25;21(1):78

13.Wei P, Yang Y, Liu Z, Luo Z, Tu W, Han J, Deng Y, Yin L*. Characterization of Autoantigen Presentation by HLA-C*06:02 in Psoriasis. J Invest Dermatol. 2017 Oct; 137(10):2238-2241

14. Zhou Z#, Lei J#*, Fang J#, Chen P, Zhou J, Wang H, Sun Z, Chen Y*, Yin L*. Dihydroartemisinin remodels tumor micro-environment and improves cancer immunotherapy through inhibiting cyclin-dependent kinases. Int Immunopharmacol. 2024 Sep 30;139:112637.

15. Sun Z, He B, Yang Z, Huang Y, Duan Z, Yu C, Dan Z, Paek C, Chen P, Zhou J, Lei J, Wang F*, Liu B*, Yin L*. Cost-Effective Whole Transcriptome Sequencing Landscape and Diagnostic Potential Biomarkers in Active Tuberculosis. ACS Infect Dis. 2024 Jun 14;10(6):2318-2332.

16. Fang J, Lei J, He B, Wu Y, Chen P, Sun Z, Wu N, Huang Y, Wei P, Yin L*, Chen Y*. Decoding the transcriptional heterogeneity, differentiation lineage, clinical significance in tissue-resident memory CD8 T cell of the small intestine by single-cell analysis. J Transl Med. 2024 Feb 25;22(1):203.

17. Lei J, Zhou Z, Fang J, Sun Z, He M, He B, Chen Q, Paek C, Chen P, Zhou J, Wang H, Tang M, Yin L*, Chen Y*. Aspirin induces immunogenic cell death and enhances cancer immunotherapy in colorectal cancer. Int Immunopharmacol. 2023 Aug;121:110350

18. Lei J, Yang Y, Lu Z, Pan H, Fang J, Jing B, Chen Y*, Yin L*. Taming metabolic competition via glycolysis inhibition for safe and potent tumor immunotherapy. Biochem Pharmacol. 2022 Aug;202:115153.

19. Liu Y, Lei J, San D, Yang Y, Paek C, Xia Z, Chen Y*, Yin L*. Structural basis for unusual TCR CDR3βusage against an immunodominant HIV-1 Gag protein peptide restricted to an HLA-B*81:01 molecule. Front. Immunol. 2022 Jan 31;13:822210

20. Yang Y，Bai H, Wu Y, Chen P, Zhou J, Lei J, Ye X, Brown A, Zhou X, Shu T, Chen Y, Wei P*,Yin L*. The Activating receptor KIR2DS2 bound to HLA-C1 reveals the novel recognition features of activating receptor. Immunology. 2022 Mar;165(3):341-354

21. Liu Y, Wu Y, Liu B, Zhang Y, San D, Chen Y, Zhou Y, Yu L, Zeng H, Zhou Y, Zhou F, Yang H, Yin L*, Huang Y*. Biomarkers and Immune Repertoire Metrics Identified by Peripheral Blood Transcriptomic Sequencing Reveal the Pathogenesis of COVID-19.Front Immunol. 2021 Aug 24;12:677025

22. Bai P*, Li Y, Zhou Q, Xia J, Wei PC, Deng H, Wu M, Chan SK, Kappler JW, Zhou Y, Tran E, Marrack P, Yin L*. Immune-based mutation classification enables neoantigen prioritization and immune feature discovery in cancer immunotherapy. Oncoimmunology. 2021 Jan 15;10(1)

23. Xia J, Bai P, FanW, Li Q, Li Y, Wang D, Yin L* and Zhou Y* NEPdb: A Database of T-Cell Experimentally-Validated Neoantigens and Pan-Cancer Predicted Neoepitopes for Cancer Immunotherapy. Front. Immunol. 2021 Apr 13;12:644637

24. Zhang L, Dong L, Lu C, Huang W, Yang C, Wang Q, Wang Q, Lei R, Sun R, Wan K, Li T, Sun F, Gan T, Lin J*, Yin L*. Methylation of SDC2/TFPI2 and Its Diagnostic Value in Colorectal Tumorous Lesions. Front Mol Biosci. 2021 Dec 22;8:706754

25. Lei J, Wang HJ, Zhu DM, Wan YB, Yin L*. Combined effects of avasimibe immunotherapy, doxorubicin chemotherapy and Metal-Organic Frameworks nano-particles on breast cancer. Journal of Cellular Physiology. 2020

26. Wei P, Song G, Shi M, Zhou Y, Liu Y, Lei J, Chen P, Yin L*. Substrate analog interaction with MCR-1 offers insight into the rising threat of the plasmid-mediated transferable colistin resistance. FASEB J. 2018 Feb; 32(2):1085-1098 27. Yin L*, Dai S, Clayton G, Gao W, Wang Y, Kappler J, Marrack P*. Recognition of self and altered self by T cells in autoimmunity and allergy. Protein & cell 2013 Jan; 4(1):8-16

28. Yin L, Crawford F, Marrack P, Kappler J*, Dai S*. T-cell receptor(TCR) interaction with peptides that mimic nickel offers insight into nickel contact allergy. PNAS（Proceedings of the National Academy of Sciences）2012 Nov 6; 109(45):18517-22

29. Yin L, Scott-Browne J, Kappler J, Gapin L, Marrack P*. T cells and their eons-old obsession with MHC. Immunological Reviews. 2012 Nov; 250(1):49-60.

30. Yin L#, Huseby E#, Scott-Browne J, Rubtsova K, Pinilla C, Crawford F, Marrack P, Dai S, Kappler J*. A Single T Cell Receptor Bound to Major Histocompatibility Complex Class I and Class II Glycoproteins Reveals Switchable TCR Conformers. Immunity. 2011 Jul 22; 35(1):23-33

31. Hu Y#, Gai Y#, Yin L#, Wang X, Feng C, Feng L, Li D, Jiang XN, Wang DC*. Crystal structures of a Populus tomentosa 4-coumarate:CoA ligase shed light on its enzymatic mechanisms. Plant Cell. 2010 Sep; 22(9):3093-104

32.殷雷etc.“第29章：免疫信息学研究进展”，《免疫学及实验技术新进展》，2018，中华医学电子音像出版社